CCHMC Genomics Sequencing Facility

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Logins to the CCHMC Genomics Sequencing Facility interface utilize CCHMC network IDs for internal investigators, or StratoCore email addresses for external investigators. Please enter the appropriate information below.

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For external (non-CCHMC) investigators, if you have forgotten your StratoCore password, you can reset it by sending an email to help-cores@bmi.cchmc.org.

For CCHMC investigators, click here to reset your password.

ABOUT THE CCHMC Genomics Sequencing Facility


  The facility is a technical and expertise resource for the genomic research community at Children's Hospital and the University of Cincinnati. We offer a wide variety of services for DNA Sequencing and Genotyping.

We are located in room 1045A of the Research Foundation (R) Building. Our regular hours of operation are 9:00 am to 5:00 pm on weekdays.

We use the ABI PRISM® 3730xl DNA Analyzer, a capillary electrophoresis system developed specifically for production-scale DNA analysis. Sequencing accuracy is > 98.5% to 550 base pairs. Read length can reach over 800 bp with good starting materials. The current capacity is around 1080 sequence reads, or over 10,000 microsatellite based genotypes per day, 7 days a week.


Instrumentation
We have recently purchased the NovaSeq X Plus system from Illumina. This is the current standard bearer in high throughput, short read NGS sequencing. It is capable of running two flow cells concurrently, each containing up to 65 whole-genome libraries at 30x coverage.

 
  The facility is also equipped with two robotic laboratory liquid handlers to automate Sanger sequencing reaction setup, dye terminator removal, Infinium wash and stain procedures for gentoyping assays and other automation.



CONTACT US


Michael Dixon
(513) 636-2801
Michael.Dixon@cchmc.org

Matt Monfils
(513) 636-2801
Matthew.Monfils@cchmc.org

Brian Quinn
(513) 636-2801
Brian.Quinn@cchmc.org

Jiyoung Kim
(513) 636-2801
Jiyoung.Kim@cchmc.org


Hassan Chaib
(513) 636-0122
Hassan.Chaib@cchmc.org

Laboratory/General
(513) 636-2801
dnacore@cchmc.org

David Fletcher
(513) 803-4897
David.Fletcher@cchmc.org

Ben Turnbull
(513) 636-2801
Benjamin.Turnbull@cchmc.org