TaqMan SNP Genotyping service | Illumina SNP Genotyping service | Submissions/Project Requests
Introduction
The facility offers multiple genotyping options for the determination of genotypes at specific positions in the genome (SNPs). We can help you determine which of the technologies we support is most appropriate for your SNP genotyping project. Number of samples to process, number of SNPs to interrogate, and the complexity of the chromosomal segment (GC content, repeats) around the SNPs of interest are all factors to consider before choosing a genotyping platform.
TaqMan SNP Genotyping service
This technology is most appropriate for projects of medium size (24 to 768 samples) requiring the genotyping of fewer than 100 SNPs.
This service begins with the review of the list of SNPs to be genotyped as not all SNPs can be typed using the TaqMan technology.
Once your list of SNPs is finalized you will need to order the SNP assays from LifeTech/ThermoFisher and we will begin the genotyping process upon receipt of the assays and your DNA.
We require 40ng of genomic DNA per SNP assay and it must be supplied at 20ng/ul in 96 well plates. A minimum of 10ul is required (200ng) regardless of the number of assays to be performed.
All samples must be at the same concentration and purity for the procedure to give best results. Purity should be assessed spectrometrically and a ratio of OD260/OD280 as close to 1.8 as possible at pH=8.5 should be obtained before submitting samples.
The facility runs the standard TaqMan protocol as recommended by the manufacturer, which is successful for the majority of the (Validated) SNP assays.
However, you may see a decrease in the call rate for a small number of "difficult" (Functionally Tested) assays. The turnaround time for TaqMan projects is usually one week for up to 1,000 genotypes once all the materials have been received.
SNP assays costs range from $200 to $375 apiece and are sufficient for processing up to 1500 samples. In addition, there is a volume dependent charge starting at $0.85 for each requested genotype.
Illumina SNP Genotyping service
The Illumina iScan platform offers great flexibility for high throughput SNP genotyping.
The Infinium assay allows the multiplexed genotyping of thousands of SNPs pre-selected across the genome for any number of samples. The different array sizes have specific applications like linkage analysis, LOH mapping, molecular karyotyping and whole genome associations. Contact us at (513) 636-2801 for Infinium genotyping pricing and currently offered arrays or visit the cytogenetics laboratory for additional information.Submission/Project Requests
Due to the nature of these SNP genotyping projects, an online submission interface has not been developed for these services. A member of the DNA Sequencing and Genotyping Core staff will make the online submission, once provided with essential information about the project. We have developed a requisition form for non-sequencing based projects here.